Conference paper
Deformation of single cells - Optical two-beam traps and more
Optofluidics, Nano and Bio-physical Systems, Department of Health Technology, Technical University of Denmark1
Nano and Bio-physical Systems, Department of Health Technology, Technical University of Denmark2
Department of Health Technology, Technical University of Denmark3
Technical University of Denmark4
Copenhagen University Hospital Herlev and Gentofte5
Nanofluidics and Bioimaging, Nano and Bio-physical Systems, Department of Health Technology, Technical University of Denmark6
Department of Physics, Technical University of Denmark7
Quantum Physics and Information Technology, Department of Physics, Technical University of Denmark8
An optical two-beam trap composed from two counter propagating laser beams is an interesting setup due to the ability of the system to trap, hold, and stretch soft biological objects like vesicles or single cells. Because of this functionality, the system was also named the optical stretcher by Jochen Guck, Josep Kaas and co-workers almost 20 years ago.
In a favorable setup, the two opposing laser beams meet with equal intensities in the middle of a fluidic channel in which cells may ow past, be trapped, stretched, and allowed to move on, giving the promise of a high throughput device. Yet, single beam optical traps, aka optical tweezers, by far outnumber the existing optical stretchers in research labs throughout the world.
The ability to easily construct an optical stretcher setup in a low-cost material would possibly imply more frequent use of the optical stretching technique. Here, we discuss advantages and disadvantages of choice of material and methodology for chip assembly and chip production. For high throughput investigations of stretching deformation of single cells, optical stretching is, however, out-performed by hydrodynamic deformability assays.
As we will discuss, injection molded polymer chips may with advantage be applied both for optical stretching and for hydrodynamic deformability experiments.
Language: | English |
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Publisher: | SPIE - International Society for Optical Engineering |
Year: | 2019 |
Pages: | 1093516-1093516-9 |
Proceedings: | Complex Light and Optical Forces XIII |
Series: | Proceedings of Spie - the International Society for Optical Engineering |
ISBN: | 1510625127 , 1510625135 , 9781510625129 and 9781510625136 |
ISSN: | 1996756x and 0277786x |
Types: | Conference paper |
DOI: | 10.1117/12.2513407 |
ORCIDs: | Nielsen, Kirstine Sandager , Marie, Rodolphe , Berg-Sørensen, Kirstine and 0000-0001-8034-1523 |