Book chapter · Journal article
Parts Characterization for Tunable Protein Expression
In
Methods in Molecular Biology
—
2018, Volume 1671, pp. 3-14
Abstract
Flow-seq combines flexible genome engineering methods with flow cytometry-based cell sorting and deep DNA sequencing to enable comprehensive interrogation of genotype to phenotype relationships. One application is to study the effect of specific regulatory elements on protein expression. Constructing targeted genomic variation around genomically integrated fluorescent marker genes enables rapid elucidation of the contribution of specific sequence variants to protein expression.
Such an approach can be used to characterize the impact of modifications to the Shine-Dalgarno sequence in Escherichia coli.
| Language: | English |
|---|---|
| Year: | 2018 |
| Pages: | 3-14 |
| Series: | Methods in Molecular Biology |
| ISBN: | 1493972944 , 1493972952 , 9781493972944 and 9781493972951 |
| ISSN: | 10643745 and 19406029 |
| Types: | Book chapter and Journal article |
| DOI: | 10.1007/978-1-4939-7295-1_1 |
| ORCIDs: | Klausen, Michael Schantz and Sommer, Morten Otto Alexander |
