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Conference paper

Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea

By Ståhl, Marie1,2,3; Hjulsager, Charlotte Kristiane2,3,4; Breum, Solvej Østergaard2,3,4; Larsen, Lars Erik2,3,4; Jorsal, Sven Erik Lind2,3,5; Angen, Øystein1,2,3

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Bacteriology & Pathology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1

Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2

National Veterinary Institute, Technical University of Denmark3

Virology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4

Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark5

At the National Veterinary Institute in Denmark we want to optimize routine diagnostic analyses by screening samples simultaneously for several agents by real time PCR. Here we present the validation of real time PCR assays for E. coli F4. E coli F18 and Lawsonia intracellularis2 in pig feces. The validation is based on feces samples spiked with a serial dilution of the respective bacteria for determination of PCR efficiencies, dynamic ranges and detection limits.

In addition, the effect of the PCR assays of different concentrations of feces and pig to pig variation have been evaluated.

Language: English
Year: 2008
Proceedings: Advances in qPCR
Types: Conference paper
ORCIDs: Hjulsager, Charlotte Kristiane and Larsen, Lars Erik
Keywords

SDG 3 - Good Health and Well-being

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