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Conference paper

Reprogramming amino acid catabolism in CHO cells with CRISPR-Cas9 genome editing improves cell growth and reduces by-product secretion

By Ley, Daniel1,2,3; Pereira, Sara2,3; Pedersen, Lasse Ebdrup2,4; Arnsdorf, Johnny2,5; Hefzi, Hooman6; Lund, Anne Mathilde7,8; Kwang Ha, Tae2,3; Wulff, Tune2,4,5; Kildegaard, Helene Faustrup2,3; Andersen, Mikael Rørdam1,9

From

Department of Biotechnology and Biomedicine, Technical University of Denmark1

Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2

CHO Cell Line Engineering and Design, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3

iLoop, Translational Management, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4

CHO Core, Translational Management, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5

University of California at San Diego6

Department of Systems Biology, Technical University of Denmark7

Network Engineering of Eukaryotic Cell Factories, Department of Systems Biology, Technical University of Denmark8

Network Engineering of Eukaryotic Cell factories, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark9

CHO cells primarily utilize amino acids for three processes: biomass synthesis, recombinant protein production and catabolism. In this work, we disrupted 9 amino acid catabolic genes participating in 7 dierent catabolic pathways, to increase synthesis of biomass and recombinant protein, while reducing production of growth-inhibiting metabolic by-products from amino acid catabolism.

Language: English
Year: 2017
Types: Conference paper
ORCIDs: Ley, Daniel , Pereira, Sara , Pedersen, Lasse Ebdrup , Arnsdorf, Johnny , Kwang Ha, Tae , Wulff, Tune , Kildegaard, Helene Faustrup and Andersen, Mikael Rørdam

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