Conference paper
Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea
Bacteriology & Pathology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Virology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark5
At the National Veterinary Institute in Denmark we want to optimize routine diagnostic analyses by screening samples simultaneously for several agents by real time PCR. Here we present the validation of real time PCR assays for E. coli F4. E coli F18 and Lawsonia intracellularis2 in pig feces. The validation is based on feces samples spiked with a serial dilution of the respective bacteria for determination of PCR efficiencies, dynamic ranges and detection limits.
In addition, the effect of the PCR assays of different concentrations of feces and pig to pig variation have been evaluated.
Language: | English |
---|---|
Year: | 2008 |
Proceedings: | Advances in qPCR |
Types: | Conference paper |
ORCIDs: | Hjulsager, Charlotte Kristiane and Larsen, Lars Erik |