Conference paper
Comparison of two quantitative PCR techniques for porcine circovirus Type 2 (PCV2) nucleic acid in field samples
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Autonomous University of Barcelona4
Section for Veterinary Epidemiology and public sector consultancy, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark5
Virology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark6
Porcine circovirus type 2 /PCV2) is considered the essential infectious agent of postweaning multisystemic wasting syndrome (PMWS), a global swine disease of devastating economic and animal welfare impact. Most pigs become infected with PCV2 during their life, but only a proportion of them develop PMWS (1).
PMWS is associated with a high PCV2 load, and a general threshold of 10 7 copies of PCV2 per ml serum has been suggested for PMWS diagnosis (2,3). The objective of this study was to compare the performance of two different real-time quantitative polymerase chain reaction (qPCR) assays for PCV2 used routinely in two laboratoires located in Denmark (lab 1) and Spain (lab 2), respectively.
| Language: | English |
|---|---|
| Year: | 2008 |
| Proceedings: | 20th International Pig Veterinary Society Congress |
| Types: | Conference paper |
| ORCIDs: | Hjulsager, Charlotte Kristiane and Larsen, Lars Erik |
