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Pedersen
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title:(Fed-batch AND production AND of AND hydrophobin AND RodB AND from AND Aspergillus AND fumigatus AND in AND host AND Pichia AND pastoris)
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Conference paper
Fed-batch production of hydrophobin RodB from Aspergillus fumigatus in host Pichia pastoris
foam. Fed-batch production yielded approximately 260 mg/L. rRodB showed good emulsifying properties. Conclusion: Hydrophobin RodB from Aspergillus fumigatus was successfully produced by yeast host Pichia pastoris in a fed-batch fermentor with good yields. Functional investigations of the hydrophobin
Year: 2011
Language: English
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Conference paper
. The expression of the RodA and RodB genes was first studied in culture flasks in buffered complex methanol medium as protein production was dependent on the methanol-induced AOX1 promoter. Later production was scaled up to a 2 L fed-batch fermentor. Hydrophobins were purified using His-select Nickel Affinity gel
Year: 2011
Language: English
